Review Form

Biohazards? No

Radioisotopes? No

Carcinogens? No

Toxic Chemicals? No

Blood and/or tissue collection, Field studies

• Blood and/or tissue collection
• Survival surgery
• Antibody production and collection
• Non-survival surgery
• Behavioral studies
• Aseptic surgery
• Prolonged physical restraint
• Multiple surgeries on the same animal
• Food or water deprivation
• Alleviated pain
• Environmental extremes
• Unalleviated pain
• Electrical stimuli
• Anesthetics used
• Induction of trauma
• Immobilizing agents without anesthesia
• Work to be done off campus
• Field studies

Animal Census and Housing

The animals will NOT be housed

Maximum number of hours at one time the animals will be kept in the laboratory/surgery: 12-24 hrs

Are prolonged (more than a few hours) physical restraint procedures part of this project? Yes

Euthansia

Will tissues, cells or other products derived from animals be shared with other investigators during or after the project? No

Are there any special requirements for animal housing, diets, restraint, or procedures for disposal? No

Lay Summary

How might a patient population (human or animal) benefit from your experiments? Provide background information on your study. Explain why it is important to do the study.

What experimental manipulations will be done with the animals (in general terms)?

Why must animals be used in these experiments and why is this particular species selected (as opposed to animals of a lower phylogeny or a non-animal alternative)?

Why are the animal model(s) you are proposing the most appropriate model(s) for achieving the objectives of this project?

How will you ensure that pain and distress will be minimized or prevented?

What happens to the animals at the end of the experiment?

Experimental Summary Upload

Instructions for disposition of sick or injured animals

Instructions for disposition of dead animals

Will animals be observed, manipulated, or trapped in the wild? No

Will you be exposing, injecting or otherwise administering to mice or rats any biological materials previously obtained from or passaged through mice or rats such as serum or cell lines (tumor cells, tissues, hybridomas, antibodies, etc.)? No

Are behavioral studies involved in this project? No

Criteria for monitoring the condition of the animals during food and water deprivation:

Will there be unrelieved pain or stress (defined as lasting for more than a moment, i.e. longer duration or more painful than a needlestick)? No

Is death an end-point? No

Assurances

Alternatives refer to methods or approaches which result in refinement of procedures which lessen pain and/or distress; reduction in numbers of animals required; or replacement of animals with non-whole-animal systems or replacement of one animal species with another, particularly if the substituted species is non-mammalian or invertebrate. I have determined that the following alternatives are available. Described briefly if applicable

test

Review Form

Biohazards? No

Radioisotopes? No

Carcinogens? No

Toxic Chemicals? No

Blood and/or tissue collection, Field studies

• Blood and/or tissue collection
• Survival surgery
• Antibody production and collection
• Non-survival surgery
• Behavioral studies
• Aseptic surgery
• Prolonged physical restraint
• Multiple surgeries on the same animal
• Food or water deprivation
• Alleviated pain
• Environmental extremes
• Unalleviated pain
• Electrical stimuli
• Anesthetics used
• Induction of trauma
• Immobilizing agents without anesthesia
• Work to be done off campus
• Field studies

Animal Census and Housing

The animals will NOT be housed

Maximum number of hours at one time the animals will be kept in the laboratory/surgery: 12-24 hrs

Are prolonged (more than a few hours) physical restraint procedures part of this project? Yes

Euthansia

Will tissues, cells or other products derived from animals be shared with other investigators during or after the project? No

Are there any special requirements for animal housing, diets, restraint, or procedures for disposal? No

Lay Summary

How might a patient population (human or animal) benefit from your experiments? Provide background information on your study. Explain why it is important to do the study.

What experimental manipulations will be done with the animals (in general terms)?

Why must animals be used in these experiments and why is this particular species selected (as opposed to animals of a lower phylogeny or a non-animal alternative)?

Why are the animal model(s) you are proposing the most appropriate model(s) for achieving the objectives of this project?

How will you ensure that pain and distress will be minimized or prevented?

What happens to the animals at the end of the experiment?

Experimental Summary Upload

Instructions for disposition of sick or injured animals

Instructions for disposition of dead animals

Will animals be observed, manipulated, or trapped in the wild? No

Will you be exposing, injecting or otherwise administering to mice or rats any biological materials previously obtained from or passaged through mice or rats such as serum or cell lines (tumor cells, tissues, hybridomas, antibodies, etc.)? No

Are behavioral studies involved in this project? No

Criteria for monitoring the condition of the animals during food and water deprivation:

Will there be unrelieved pain or stress (defined as lasting for more than a moment, i.e. longer duration or more painful than a needlestick)? No

Is death an end-point? No

Assurances

Alternatives refer to methods or approaches which result in refinement of procedures which lessen pain and/or distress; reduction in numbers of animals required; or replacement of animals with non-whole-animal systems or replacement of one animal species with another, particularly if the substituted species is non-mammalian or invertebrate. I have determined that the following alternatives are available. Described briefly if applicable

test

Review Form

Biohazards? No

Radioisotopes? No

Carcinogens? No

Toxic Chemicals? tunicamycin, thapsigargin, sodium arsenite,

Blood and/or tissue collection, Survival surgery, Behavioral studies, Anesthetics used

• Blood and/or tissue collection
• Survival surgery
• Antibody production and collection
• Non-survival surgery
• Behavioral studies
• Aseptic surgery
• Prolonged physical restraint
• Multiple surgeries on the same animal
• Food or water deprivation
• Alleviated pain
• Environmental extremes
• Unalleviated pain
• Electrical stimuli
• Anesthetics used
• Induction of trauma
• Immobilizing agents without anesthesia
• Work to be done off campus
• Field studies

Animal Census and Housing

Maximum number of hours at one time the animals will be kept in the laboratory/surgery: 0-12 hrs

Is surgery involved in the proposed project? Yes

Are stressful or painful procedures, other than surgery, part of this project? Yes

Will genetically engineered or other mutant animals be produced or maintained that may be expected to develop clinical signs as a result of the mutation? Yes

Euthansia

Will tissues, cells or other products derived from animals be shared with other investigators during or after the project? No

Are there any special requirements for animal housing, diets, restraint, or procedures for disposal? Yes

Explanation(s) for each parameter involved:

Adult zebrafish will be maintained on a 14:10 light–dark cycle and fed three times daily.

Lay Summary

How might a patient population (human or animal) benefit from your experiments? Provide background information on your study. Explain why it is important to do the study.

What experimental manipulations will be done with the animals (in general terms)?

Why must animals be used in these experiments and why is this particular species selected (as opposed to animals of a lower phylogeny or a non-animal alternative)?

Why are the animal model(s) you are proposing the most appropriate model(s) for achieving the objectives of this project?

How will you ensure that pain and distress will be minimized or prevented?

What happens to the animals at the end of the experiment?

Experimental Summary Upload

Instructions for disposition of sick or injured animals

Instructions for disposition of dead animals

Will animals be observed, manipulated, or trapped in the wild? No

Will you be exposing, injecting or otherwise administering to mice or rats any biological materials previously obtained from or passaged through mice or rats such as serum or cell lines (tumor cells, tissues, hybridomas, antibodies, etc.)? No

Are behavioral studies involved in this project? Yes

Criteria for monitoring the condition of the animals during food and water deprivation: N/A.

Will there be unrelieved pain or stress (defined as lasting for more than a moment, i.e. longer duration or more painful than a needlestick)? No

Is death an end-point? No

Assurances

Alternatives refer to methods or approaches which result in refinement of procedures which lessen pain and/or distress; reduction in numbers of animals required; or replacement of animals with non-whole-animal systems or replacement of one animal species with another, particularly if the substituted species is non-mammalian or invertebrate. I have determined that the following alternatives are available. Described briefly if applicable

N/A.

SUPPLEMENTAL FORM(S) ARE REQUIRED